Analytical method development of Darunavir and Ropivacain as bulk drug and formulation using chromogenic reagents

Simple and rapid spectrophotometric & chromatographic methods have been developed and validated for the determination of Darunavir and Ropivacain in tablets and as bulk drug. The methods applied were involing use 1,2-naphthoquinone-4-sulphonate (NQS). The formar was solubilised in an alkaline medium with NQS to form an orange-colored product with λmax at 488 nm while the later gave blue colored chromogen with λmax at 565 nm. The chromogen obeyed Beer’s law in the concentration range of 5-60μg/mL. The results of the analysis have been validated statistically and by recovery studies.